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    • Coffee Leaf Rust Photos
    • HDOA CLR Pest Alert >
      • HDOA CLR Report Form
    • Sanitation and Disinfestation Info
    • Surveying, Sampling and Monitoring of CLR >
      • HDOA CLR Field Guide and Submission Form
    • Spraying for CLR and Spray Product Info >
      • Spraying for CLR - English
      • Pulverización para CLR - Español
      • Spraying for CLR - Tagalog
      • Spraying for CLR - Ilocano
      • Sprayer Calibration and Pesticide Calculations
      • Priaxor Xemium Info
      • ProBlad Verde Info
      • Pesticide Use FAQs
    • Pruning for CLR and CBB
    • Coffee Leaf Rust Poster >
      • La Roya del Café Póster - Español
      • Coffee Leaf Rust Poster - Tagalog
      • Coffee Leaf Rust Poster - Ilocano
    • CLR Trifold Brochure
    • CLR Presentations and Meetings
    • CLR Publications
    • CLR/CBB Subsidy Program Info
    • Bioworks BotaniGard and Mycotrol Compatibility Chart
  • CBB Management
    • Field Sanitation
    • Pruning >
      • Two-Year Cycle Block Pruning Method by Aki Yamagishi
    • Feral and Unmanaged Coffee Trees
    • Field Monitoring
    • CBB Control >
      • Predators of CBB
    • Harvesting
    • Shipping
    • Beauveria Subsidy Program Info
    • Bioworks BotaniGard and Mycotrol Compatibility Chart
    • 2020 CBB IPM Document
    • CBB & Coffee Presentations & Posters
    • CBB Publications
  • Events and Announcements
    • 4/11-4/15 Sprayer Calibration and Respirator Use and Fit Testing Workshops - Kona, Hilo & Kau
    • Feb/Mar CLR Research and Management Update Webinars with Live Q&A
    • Respirator Fit Test Training Workshops - Sign Up
    • Hands-on Coffee Grafting Workshops - Sign Up
  • Hawaii Coffee Pests and Problems
    • Anthracnose
    • Banana Moth
    • Bird Damage
    • Black Twig Borer
    • Blister Spot
    • Cercospora Leaf Spot and Berry Blotch
    • Coffee Berry Borer
    • Coffee Root-knot Nematode
    • Food Safety Concerns - Slug, feces, etc.
    • Fruit Fly
    • Glyphosate Herbicide Injury
    • Green Scale and Sooty Mold
    • J-rooting
    • Little Fire Ant
    • Mite Feeding Damage
    • Nutritional Problems
    • Over-bearing Dieback
    • Rodent Damage
    • Skin Cracking
    • Slug and Snail Damage
    • Sunburn on Coffee
  • NKO - Coffee Pests and Diseases Not in Hawaii
    • Coffee Berry Disease - NKO
    • Coffee Leaf Miner - NKO
    • Coffee White Stem Borer - NKO
    • Coffee Wilt Disease - NKO
  • Coffee Publications and Presentations
    • Growing Coffee in Hawaii
  • Coffee Leaf and Soil Sampling Instructions
  • HDOA Rules - Coffee
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    • Chapter 145 - Reg. of Farm Product
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Sample Submissions to UH ADSC
​(11/6/20)


ADSC instructions
To submit a leaf sample for coffee leaf rust ID, follow the instructions below.
 
Supplies:
  • Clean, unused Ziplock bag(s)
  • Permanent marker
  • Clean, unused paper towels
  • Cooler with a small bag of ice, an ice pack, and a small, light towel for keeping the samples cool
  • >70% alcohol
  • Optional: Gloves
 
Procedure:
  1. Organize a cooler before collecting the leaf samples.
    • Place a small bag of ice or an icepack on the bottom of the cooler and cover the ice or icepack with a light towel.
  2. Clearly label the clean, unused Ziploc bag(s) with:
    • Your name and/or farm name, 
    • Date of collection, and 
    • Field or section ID from where the sample is to be taken. 
  3. Collect coffee leaves that display coffee leaf rust (CLR) symptoms. 
    • Photos of CLR can be found at: https://www.hawaiicoffeeed.com/clr.html
    • Optional: use gloves to collect the leaf samples.
    • Try to collect leaves that do not have water, dew, or other free forms of water on them, or rot may set in.
    • If leaves have water and other moisture on them and you must submit these leaves, DO NOT wipe off the water. Gently shake off any excess water and place the leaves between two sheets of clean paper towel. 
    • Place the leaves in the bag and seal the bag completely.
  4. Sanitize your hands or gloves, the outside of the Ziplock(s), and any tools prior to the next sample collection within the farm. 
    • Follow sanitation protocols prior to driving to another location or farm. 
    • https://www.hawaiicoffeeed.com/clr-sanitation-protocol.html
  5. Place the bagged leaf sample(s) ON the towel for indirect cooling and to preserve the integrity of the sample. 
    • OR, place the bagged leaf samples in a refrigerator. 
  6. Do not freeze samples. 
  7. Prevent decay and rot of leaves. Submit tissue samples as soon as possible.  
  8. Bring the sample to your nearest Cooperative Extension Office on Mondays or Tuesday mornings before 10:00 am. 
    • It may be best to schedule an appointment to be sure that staff are able to assist you and accept your sample and payment. Locate the various Cooperative Extension offices and contact information at: https://cms.ctahr.hawaii.edu/ce/Find-Us
  9. Fill out the proper forms with assistance from the office staff. 
    • Include your farm address and TMK on the forms to assist with survey records and farm assistance.
    • Include an email or phone number for communications.
  10. Pay for the sample analysis. Cash (in full amount) and check (to: University of Hawaii) is accepted. 
    • Disease (D1) samples cost $12 a sample. 
    • Visit https://www.ctahr.hawaii.edu/site/downloads/adsc/price_list.pdf to view all ADSC analytical service fees.
    • When completed, the results will be sent to you via email and/or mail. 
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  • Home
  • Coffee Leaf Rust
    • Coffee Leaf Rust Photos
    • HDOA CLR Pest Alert >
      • HDOA CLR Report Form
    • Sanitation and Disinfestation Info
    • Surveying, Sampling and Monitoring of CLR >
      • HDOA CLR Field Guide and Submission Form
    • Spraying for CLR and Spray Product Info >
      • Spraying for CLR - English
      • Pulverización para CLR - Español
      • Spraying for CLR - Tagalog
      • Spraying for CLR - Ilocano
      • Sprayer Calibration and Pesticide Calculations
      • Priaxor Xemium Info
      • ProBlad Verde Info
      • Pesticide Use FAQs
    • Pruning for CLR and CBB
    • Coffee Leaf Rust Poster >
      • La Roya del Café Póster - Español
      • Coffee Leaf Rust Poster - Tagalog
      • Coffee Leaf Rust Poster - Ilocano
    • CLR Trifold Brochure
    • CLR Presentations and Meetings
    • CLR Publications
    • CLR/CBB Subsidy Program Info
    • Bioworks BotaniGard and Mycotrol Compatibility Chart
  • CBB Management
    • Field Sanitation
    • Pruning >
      • Two-Year Cycle Block Pruning Method by Aki Yamagishi
    • Feral and Unmanaged Coffee Trees
    • Field Monitoring
    • CBB Control >
      • Predators of CBB
    • Harvesting
    • Shipping
    • Beauveria Subsidy Program Info
    • Bioworks BotaniGard and Mycotrol Compatibility Chart
    • 2020 CBB IPM Document
    • CBB & Coffee Presentations & Posters
    • CBB Publications
  • Events and Announcements
    • 4/11-4/15 Sprayer Calibration and Respirator Use and Fit Testing Workshops - Kona, Hilo & Kau
    • Feb/Mar CLR Research and Management Update Webinars with Live Q&A
    • Respirator Fit Test Training Workshops - Sign Up
    • Hands-on Coffee Grafting Workshops - Sign Up
  • Hawaii Coffee Pests and Problems
    • Anthracnose
    • Banana Moth
    • Bird Damage
    • Black Twig Borer
    • Blister Spot
    • Cercospora Leaf Spot and Berry Blotch
    • Coffee Berry Borer
    • Coffee Root-knot Nematode
    • Food Safety Concerns - Slug, feces, etc.
    • Fruit Fly
    • Glyphosate Herbicide Injury
    • Green Scale and Sooty Mold
    • J-rooting
    • Little Fire Ant
    • Mite Feeding Damage
    • Nutritional Problems
    • Over-bearing Dieback
    • Rodent Damage
    • Skin Cracking
    • Slug and Snail Damage
    • Sunburn on Coffee
  • NKO - Coffee Pests and Diseases Not in Hawaii
    • Coffee Berry Disease - NKO
    • Coffee Leaf Miner - NKO
    • Coffee White Stem Borer - NKO
    • Coffee Wilt Disease - NKO
  • Coffee Publications and Presentations
    • Growing Coffee in Hawaii
  • Coffee Leaf and Soil Sampling Instructions
  • HDOA Rules - Coffee
  • USDA - Coffee Rules
  • Coffee Organizations in Hawaii
  • Agricultural Theft
    • Chapter 145 - Reg. of Farm Product
    • Dealer Licensing
    • Contacts
  • Coffee Crop Insurance
  • Archive
  • About us
  • Blog